Components Available For Mouse on Mouse IHC
Mouse-on-Mouse Biotinylation Kit
Mouse monoclonal antibodies can be used for immunohistochemical detection on mouse tissues; however, the biotinylated secondary antibody used for detection will bind to the primary antibody and to the endogenous mouse IgG in the tissue resulting in background staining from bound mouse IgG. To circumvent the problem, BIOCARE has developed the MM Biotinylation Kit. The kit provides all the reagents necessary to biotinylate a primary antibody.
The basic concept of the kit is very simple. The antibody is mixed with a biotinylation reagent for 30 minutes, prior to the application of primary antibody. As little as 5 to 10 UL of antibody can be used. Once the complex is formed, a Mopping Reagent is added and then the biotinylated antibody complex is applied to the tissue specimen. The tissue is then incubated with streptavidin-peroxidase, and the color reaction is developed by a DAB chromogen-substrate.
The system has been specially formulated to eliminate endogenous mouse IgG background staining. Even the most difficult tissues such as lymph, spleen, lung and kidney tissues are virtually background free.
Cat. No. MMBK, G, H, available in 6 and 25 ml
(Left) Mouse Anti-PCNA on mouse spleen.
(Right) Mouse Anti-Actin on mouse pancreas.
Vulcan Fast Red Chromogen Kit 2
Fast Red is a widely used chromogen for immunohistochemical staining. When in the presence of alkaline phosphatase (AP) enzyme, Vulcan Fast Red produces a bright fuchsin-red precipitate that is insoluble in organic solvents---and can be coverslipped with a permanent mounting media. This product is available in a stable two-component system, consisting of Vulcan Fast Red Chromogen and Vulcan Buffer.
| Name | Cat. No. | Vol. | Description |
| Vulcan Fast Red 2 | FR805 H, S, M | 25, 100, 500 ml | Chromogen Kit |
