Biocare Medical VP Echelon
Prediluted antibodies are optimized for the Ventana® Benchmark® XT Immunohistochemistry Staining Systems
The "VP-Echelon Series" from Biocare Medical . . . These conveniently packaged reagents are ready for loading into reagent dispensers supplied by Ventana. The antibodies have been titered and quality controlled to assure consistent performance and high quality staining. Biocare provides ready-to-use antibodies in 5.0 ml (50 test) and 25 ml (250 test) formats. The end user can choose the most appropriate volume for their laboratory workflow.
| ADH-5 (Breast Marker Cocktail) |
| Clone: XM26, LL002, BC4A4, BC1 and E431-1 |
Source: Mouse and Rabbit monoclonal |
DATA SHEET |
| Cat. No.: VP 360DSK |
Control: Breast Cancer |
| Pretreatment: CC1 |
| AFP |
| Clone: Polyclonal |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP028* (No VP Equivalent) |
Control: Hepatocytes of fetal liver or hepatoma |
| Pretreatment: See Below |
| Calretinin |
| Clone: Polyclonal |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP092* (No VP Equivalent) |
Control: Mesothelioma |
| Pretreatment: See Below |
| Carcinoembryonic Antigen (CEA) |
| Clone: COL-1 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP058 |
Control: Colon carcinoma |
| Pretreatment: Not required |
| CEA {P} |
| Clone: N/A |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP009* (No VP Equivalent) |
Control: Colon carcinoma |
| Pretreatment: See Below |
| Cat Scratch |
| Clone: H2A10 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM144* (replaces catalog number VP144) |
Control: B henselae infected lymph node |
| Pretreatment: See Below |
| CD3 (M) |
| Clone: PS1 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP110 |
Control: Tonsil or T-cell lymphoma |
| Pretreatment: BORG (Mouse Signal Enhancement Kit required) |
| CD5 (M) |
| Clone: 4C7 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM099* (replaces catalog number VP099) |
Control: Mantle cell lymphoma or tonsil |
| Pretreatment: See Below |
| CD5 |
| Clone: SP19 |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PRM328* (replaces catalog number VP099) |
Control: Mantle cell lymphoma or tonsil |
| Pretreatment: See Below |
| CD7 |
| Clone: LP15 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM158* (replaces catalog number VP099) |
Control: Tonsil |
| Pretreatment: See Below |
| CD10 |
| Clone: 56C6 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP129 |
Control: Tonsil, kidney, lymphoblastic leukemia |
| Pretreatment: BORG |
| CD15 |
| Clone: MMA |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM029* (No VP Equivalent) |
Control: Reed-Sternberg cells (Hodgkin's) |
| Pretreatment: See Below |
| CD15 Cocktail |
| Clone: DT07 + BC97 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP073 |
Control: Reed-Sternberg cells |
| Pretreatment: BORG or BORG + Protease II or III |
| CD20 |
| Clone: L26 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM004* (No VP Equivalent) |
Control: Tonsil or B-cell lymphoma |
| Pretreatment: See Below |
| CD30 Cocktail |
| Clone: DT05 + BC98 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP074 |
Control: Hodgkin's or anaplatic large cell lymphoma |
| Pretreatment: BORG or BORG + Protease II or III |
| CD43 |
| Clone: DF-T1 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM005* |
Control: Tonsil or T-cell lymphoma |
| Pretreatment: See Below |
| CD44 |
| Clone: 156-3C11 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM318* |
Control: Tonsil or breast cancer |
| Pretreatment: See Below |
| CD45RO |
| Clone: UCHL-1 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM006* (No VP Equivalent) |
Control: Tonsil or T-cell lymphoma |
| Pretreatment: See Below |
| CD57 |
| Clone: NK1 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM007* (No VP Equivalent) |
Control: Single Tonsil |
| Pretreatment: See Below |
| CD99 |
| Clone: HO36-1.1 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM008* (No VP Equivalent) |
Control: Pancreas or Ewing's sarcoma |
| Pretreatment: See Below |
| CD117 C-Kit (Rabbit monoclonal) |
| Clone: Y145 |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PME296* (replaces catalog number VPE296) |
Control: Skin (mast cells), GIST or seminoma |
| Pretreatment: See Below |
| CDX2 |
| Clone: CDX2-88 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP226 |
Control: Colon cancer |
| Pretreatment: BORG |
| CK5 |
| Clone: XM26 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM234* (No VP Equivalent) |
Control: Normal prostate |
| Pretreatment: See Below |
| CK6 |
| Clone: LHK6B |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM044* (No VP Equivalent) |
Control: Skin |
| Pretreatment: See Below |
| CK7 |
| Clone: OVTL 12/30 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM061* (No VP Equivalent) |
Control: Ovarian or breast cancer |
| Pretreatment: See Below |
| CK14 |
| Clone: LL02 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM185* (No VP Equivalent) |
Control: Skin or squamous cell carcinoma |
| Pretreatment: See Below |
| CK20 |
| Clone: Ks20.8 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM062* (No VP Equivalent) |
Control: Colon carcinoma |
| Pretreatment: See Below |
| Chromogranin A |
| Clone: LK2H10 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM010* (replaces catalog number VP010) |
Control: Pancreas, adrenal gland, bowel, thyroid |
| Pretreatment: See Below |
| Cyclin D1 (SP4) |
| Clone: SP4 |
Source: Rabbit Monoclonal |
DATA SHEET |
| Cat. No.: PRM307* (replaces catalog number VP307) |
Control: Mantle cell lymphoma or breast cancer |
| Pretreatment: See Below |
| Cytokeratin 7 (CK7) |
| Clone: OV-TL 12/30 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM061* (replaces catalog number VP061) |
Control: Ovarian or breast cancer |
| Pretreatment: See Below |
| Cytokeratin 20 (CK20) |
| Clone: Ks20.8 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM062* (replaces catalog number VP062) |
Control: Colon carcinoma |
| Pretreatment: Protease I |
| Cytokeratin 5/6 (CK5/6) |
| Clone: CK5/6.007 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP105 |
Control: Mesothelioma, squamous cell lung carcinoma and skin |
| Pretreatment: BORG + Protease II or III |
| D2-40 |
| Clone: D2-40 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM266* (No VP Equivalent) |
Control: Breast or colon cancer |
| Pretreatment: See Below |
| Desmin |
| Clone: D33 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM036* (replaces catalog number VP036) |
Control: Leiomyosarcoma |
| Pretreatment: See Below |
| EMA |
| Clone: Mc-5 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM143* (No VP Equivalent) |
Control: Breast carcinoma |
| Pretreatment: See Below |
| Epidermal Growth Factor Receptor (EGFR) |
| Clone: 111.6 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP063 |
Control: Squamous cell carcinoma or metastic colon cancer |
| Pretreatment: Protease I |
| Factor XIIIa |
| Clone: E980.1 |
Source: Mouse monoclonal |
DATA SHEET |
| Cat. No.: VP357 |
Control: Capillary hemangioma, dermatofibroma, placenta or skin |
| Pretreatment: NA |
| Gross Cystic Disease Fluid Protein-15 (GCDFP-15) |
| Clone: D6 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM113* (replaces catalog number VP113) |
Control: Breast cancer |
| Pretreatment: See Below |
| GFAP {M} |
| Clone: GA-5 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM065* (No VP Equivalent) |
Control: Normal brain or astrocytoma |
| Pretreatment: See Below |
| GFAP {P} |
| Clone: N/A |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP040* (No VP Equivalent) |
Control: Normal brain or astrocytoma |
| Pretreatment: See Below |
| Helicobacter pylori |
| Clone: Polyclonal |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP120* (replaces catalog number VP120) |
Control: Infected tissue from the antrum and body of the stomach |
| Pretreatment: See Below |
| Hepatocyte Specific Antibody (HSA) |
| Clone: OCH1E5 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM166* (replaces catalog number VP166) |
Control: Liver or liver carcinoma |
| Pretreatment: See Below |
| Kappa Light Chain (M) |
| Clone: N/A |
Source: Mouse Monoclonal |
DATA SHEET |
| Cat. No.: VP012 |
Control: Tonsil |
| Pretreatment: CC1 |
| Kappa (P) |
| Clone: N/A |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP013 |
Control: Tonsil |
| Pretreatment: See Below |
| Lambda Light Chain |
| Clone: LcN-2 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP014 |
Control: Tonsil |
| Pretreatment: Reveal |
| Lambda {P} |
| Clone: N/A |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP015 |
Control: Tonsil |
| Pretreatment: See Below |
| MART-1 Cocktail |
| Clone: M2-7C10 + M2-9E3 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM077 (replaces catalog number VP077) |
Control: Melanoma |
| Pretreatment: See below |
| Melanoma Cocktail |
| Clone: HMB45 + M2-7C10 + M2-9E3 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP078 |
Control: Melanoma |
| Pretreatment: BORG (an avidin-biotin block may be required) |
| Myeloperoxidase |
| Clone: N/A |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP023 |
Control: Any tissue with inflammatory process, such as colon cancer or tonsil |
| Pretreatment: See Below |
| Neurofilament |
| Clone: 2F11 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM066* (No VP Equivalent) |
Control: Normal Brain |
| Pretreatment: See Below |
| Oct 3/4 |
| Clone: SEMGC |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM313* (No VP Equivalent) |
Control: Seminoma |
| Pretreatment: See Below |
| P504S |
| Clone: Polyclonal |
Source: Rabbit |
DATA SHEET |
| Cat. No.: VP200R |
Control: Prostate carcinoma |
ASR |
| Pretreatment: Reveal (an avidin-biotin block may be required) |
| p63 |
| Clone: BC4A4 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP163 |
Control: Normal prostate |
| Pretreatment: BORG (an avidin-biotin block may be required) |
| Pan Cytokeratin [AE1 / AE3] |
| Clone: AE1 / AE3 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP011 |
Control: Skin or adenocarcinoma |
| Pretreatment: V-Trypsin |
| Pan Cytokeratin [Lu-5] |
| Clone: Lu-5 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP043 |
Control: Skin or adenocarcinoma |
| Pretreatment: Protease I |
| Pan Melanoma Cocktail (HMB45 + MART-1 + Tyrosinase) |
| Clone: HMB45 + M2-9E3 + T311 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP165 |
Control: Metastic melanoma in lymph node |
| Pretreatment: BORG or Reveal |
| Pan Mucin |
| Clone: b-12 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM139* (No VP Equivalent) |
Control: Breast cancer |
| Pretreatment: See Below |
| PCNA |
| Clone: PC10 |
Source: Mouse |
DATA SHEET |
| Cat. No.: PM152* (No VP Equivalent) |
Control: Breast or colon cancer |
| Pretreatment: See Below |
| PIN Cocktail (p63 + P504S) |
| Clone: BC4A4 + Polyclonal |
Source: Mouse + Rabbit |
DATA SHEET |
| Cat. No.: VP201 |
Control: Prostatic intraepithelial neoplasia |
|
| Pretreatment: BORG (an avidin-biotin block may be required) |
| S100 {P} |
| Clone: N/A |
Source: Rabit |
DATA SHEET |
| Cat. No.: PP021* (No VP Equivalent) |
Control: Melanoma and Schwannoma |
| Pretreatment: See Below |
| SMA |
| Clone: 1A4 (also known as asm-1) |
Source: Mouse monoclonal |
DATA SHEET |
| Cat. No.: PP001* (No VP Equivalent) |
Control: Blood vessels, leiomyoma or leiomyosarcoma |
| Pretreatment: See Below |
| Spirochete (Treponema pallidum) |
| Clone: Polyclonal |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP135* (replaces catalog number VP135) |
Control: Spirochete infected tissues |
| Pretreatment: See Below |
| TB |
| Clone: N/A |
Source: Rabbit |
DATA SHEET |
| Cat. No.: PP140 |
Control: Mycobacterium tuberculosis infected tissue |
| Pretreatment: See Below |
| TTF-1 |
| Clone: 8G7G3/1 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP087 |
Control: Lung adenocarcinoma or thyroid |
| Pretreatment: BORG |
| Villin |
| Clone: 1D2C3 |
Source: Mouse |
DATA SHEET |
| Cat. No.: VP094 |
Control: xxx |
| Pretreatment: BORG (an avidin-biotin block may be required) |
Run conditions for prediluted antibodies on the Ventana Benchmark® XT with the iVIEW detection system
| Cat. No. |
Product Name |
CC1 Mild |
CC1 Std |
CC1 Ext |
Enzyme |
No Preatreatment |
Time |
Temp |
Amp |
A/B Block |
| PP028 |
AFP |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM144 |
Cat Scratch Fever |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PME296 |
CD117 |
|
X |
|
|
|
32 - 44 |
37°c |
|
|
| PM029 |
CD15 |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM004 |
CD20 |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM005 |
CD43 |
X |
|
|
|
|
16 Min |
37°c |
|
|
| PM318 |
CD44 |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PM006 |
CD45RO |
X |
|
|
|
|
16 Min |
37°c |
|
|
| PM099 |
CD5 |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PRM328 |
CD5 |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM007 |
CD57 |
|
X |
|
|
|
32 - 44 |
37°c |
|
|
| PM158 |
CD7 |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PM008 |
CD99 |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PP009 |
CEA {P} |
|
|
|
|
X |
32 Min |
37°c |
|
|
| PM010 |
Chromogranin A |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PM185 |
CK14 |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PM062 |
CK20 |
|
|
|
ProteaseI for 4 mins |
|
32 Min |
37°c |
|
|
| PM234 |
CK5 |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM044 |
CK6 |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM061 |
CK7 |
|
|
|
ProteaseI for 8 mins |
|
32 Min |
37°c |
|
|
| PM266 |
D2-40 |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM036 |
Desmin |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM143 |
EMA |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PM113 |
GCDFP-15 |
|
|
|
ProteaseI for 8 mins |
|
32 Min |
37°c |
|
|
| PM065 |
GFAP {M} |
|
X |
|
|
|
20 Min |
37°c |
|
|
| PP040 |
GFAP {P} |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PP120 |
H. Pylori |
X |
|
|
|
|
32 Min |
37°c |
|
X |
| PM166 |
Hepatocyte Specific Antibody (HSA) |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PP013 |
Kappa {P} |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PP015 |
Lambda {P} |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PP023 |
Myeloperoxidase |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM 077 |
MART-1 Cocktail |
|
X |
|
|
|
32-44 Min |
37°c |
|
|
| PM066 |
Neurofilament |
|
X |
|
|
|
20 Min |
37°c |
|
|
| PM313 |
Oct 3/4 |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PM139 |
Pan Mucin |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM152 |
PCNA |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PP021 |
S100 {P} |
X |
|
|
|
|
32 Min |
37°c |
|
|
| PM001 |
SMA |
|
|
|
|
X |
32 Min |
37°c |
|
|
| PP135 |
Spirochete |
|
X |
|
|
|
32 Min |
37°c |
|
|
| PP140 |
TB |
|
X |
|
|
|
32 Min |
37°c |
X |
|
The protocols above must be validated in each laboratory
*These Biocare 4Plus™ predilute antibodies provide excellent IHC staining when using Ventana Benchmark instruments.
VP-Echelon antibodies are developed solely by Biocare Medical and do not imply approval or endorsement of Biocare’s antibodies by Ventana Medical Systems, Inc. Biocare and Ventana are not affiliated, associated or related in any way. Ventana®, ES®, NexES®, Benchmark® XT are trademarks of Ventana Medical Systems, Inc.
